Transient accumulations of cyanophycin in Anabaena cyZidkica and Syitechocystis 6308
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Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308.
The effect of a number of conditions on the amount of cyanophycin granule polypeptide [multi-L-arginyl poly(L-aspartic acid)] formed in the unicellular cyanobacterium Aphanocapsa 6308 was determined. Light, CO2, sulfur, and phosphorus starvation as well as the addition of arginine to culture media increased the amount of cyanophycin granule polypeptide in cells when compared with that in cells ...
متن کاملEstablishment of cyanophycin biosynthesis in Pichia pastoris and optimization by use of engineered cyanophycin synthetases.
Two strains of the methylotrophic yeast Pichia pastoris were used to establish cyanophycin (multi-L-arginyl-poly-L-aspartic acid [CGP]) synthesis and to explore the applicability of this industrially widely used microorganism for the production of this polyamide. Therefore, the CGP synthetase gene from the cyanobacterium Synechocystis sp. strain PCC 6308 (cphA(6308)) was expressed under the con...
متن کاملMeasurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica.
An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the cyanophycin granule is a cellular nitrogen reserve.
متن کاملCompartmentalized cyanophycin metabolism in the diazotrophic filaments of a heterocyst-forming cyanobacterium.
Heterocyst-forming cyanobacteria are multicellular organisms in which growth requires the activity of two metabolically interdependent cell types, the vegetative cells that perform oxygenic photosynthesis and the dinitrogen-fixing heterocysts. Vegetative cells provide the heterocysts with reduced carbon, and heterocysts provide the vegetative cells with fixed nitrogen. Heterocysts conspicuously...
متن کاملCyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine.
The cyanophycin granules from two-week-old cultures of the blue-green alga Anabaena cylindrica were isolated by sonication of the cells followed by differential centrifugation. These particles are composed of polypeptides containing only two amino acids, aspartic acid and arginine, and have a molecular weight between about 25,000 and 100,000.
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تاریخ انتشار 1990